Biological Reagents & Biological Buffers
Biological buffers are organic substances that optimize biochemical processes by maintaining the body’s correct pH. This is made possible by their neutralizing effect on hydrogen ions, which allows them to maintain a constant pH.
At ReAgent, we manufacture biological reagents for use in life science laboratories and in IVD (in vitro diagnostics) kits. Products we supply include biological buffers for medical devices used in cancer diagnoses, test kits, and DNA sample preparation.
All of our buffers and reagents are manufactured using chemicals approved for their intended use, with full change control and notification processes in place. Cur biological solutions are also subject to extensive quality checks.
Additionally, we are able to pack your buffers and reagents in sterile, DNAse and RNAse-free packaging and manufacture products in ISO Class 7 cleanroom conditions. Products are routinely filtered to 0.2µ during the packing process.
We are also able to manufacture and pack biological buffers and biological reagents that have a short shelf life, as we have both cold and frozen storage capabilities.
That means we can store and transport your products at the specified temperature to ensure they don’t degrade during storage or delivery.
We can also work with you to create a new formulation for a biological buffer, or we can support you in making changes to existing formulations.
Whatever your unique requirements, you can feel confident that you will receive a high-quality product and outstanding customer service from an expert biological buffer manufacturer.
Custom Buffer Solutions
At ReAgent, we work with you to bring your custom buffer solutions to life. We can create a new formulation for a biological buffer or support you in making changes to existing formulations.
As a customer-led business, we can adapt to meet your exact requirements. We’ll work closely with you to supply the custom buffer solutions and bioprocessing reagents you need, in the volume you need, when you need them. Our expert technical team is also on hand to provide their advice and expertise every step of the way.
Whatever your unique requirements, you can feel confident that you’ll receive a high-quality product and outstanding customer service from an expert biological buffers supplier.
Examples of Biological Buffers
There’s a wide range of biological buffers manufactured today. Different buffers will have a slightly different pH range, which is the range in which they’re able to continue resisting pH changes.
For example, while the MES buffer has a useful pH range between 5.5–6.7, HEPES buffer ranges between 6.8–8.2. Some buffers have much more basic pH ranges, like CABS buffer, which is useful between 10.0–11.4.
Ready to discuss your requirements?
Call our technical sales team at 321-REAGENT
Good’s Buffers
There are a total of twenty Good’s buffers. These were prepared and tested by Norman Good and his team between 1966 and 1980, and provided biologists with access to safe and effective buffer systems. The properties that unite all Good’s buffers include:
- High solubility in water and minimal solubility in organic solvents
- Low level of permeability to cell membranes
- Stable and consistent dissociation constants
- Low metal chelating capability
- High chemical stability
- Not toxic towards cells
- Not affected by ionic composition or temperature
- Not very absorptive of UV spectra
We can manufacture and supply a range of the twenty Good’s buffering agents used for biochemical and biological research. Some of the Good’s buffers we supply include:
Bis-Tris Buffer
Bis-tris, otherwise known as bis-Tris methane, is a protein and nucleic acid buffer used in molecular biology and biochemistry. It’s best for a pH range of 5.8-7.2 and acts as a substitute for cacodylate, a highly toxic buffer.
Bis-tris is a popular zwitterionic biological buffer solution that can be used:
- As a buffer for anion exchange chromatography
- In electrophoresis as either a running buffer, gel buffer, or sample buffer
PIPES Buffer
PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)) is another of the Good’s buffers and is typically used in biochemistry, specifically for:
- Cell culture
- Chromatography
- Cosmetics
- Diagnostic tests
- Protein purification
- Electron microscopy study
Tris Buffer
Tris (tris(hydroxymethyl)aminomethane) is widely used in molecular biology and biochemistry. It has a pH range between 7.1 and 9.1 at room temperature, which matches the physiological pH common to most living organisms.
This buffer solution is used during the pH-sensitive DNA extraction process, where it helps stabilize the pH over cell lysis and extraction.
HEPES Buffer
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulphonic acid buffer, and is one of the twenty Good’s buffers. HEPES is commonly used in cell culture and is best suited for microbiology, histology, and diagnostic laboratory procedures.
While HEPES buffer is better than bicarbonate buffers at maintaining the physiological pH of cells, it has one big drawback: it produces hydrogen peroxide when exposed to light. To mitigate risks to cells, HEPES buffer solution must be stored in darkness.
Phosphate-Buffered Saline (PBS)
Phosphate-buffered saline is a water-based salt solution frequently used in biological research for two reasons. Firstly, this buffer helps to sustain a constant pH, and secondly, the ion concentrations match the human body. This makes it well-suited for use in biological research.
As an added-value service from ReAgent, we are able to have PBS sterilized by gamma radiation post-packing. This extends the shelf life of the buffer and provides a better quality product.
Tris Hydrochloride (Tris HCl) Buffer
Tris HCl is used in pharmaceutical and biopharmaceutical production and is suitable for cell culture. It’s very water-soluble and is useful in the 7.0–9.0 pH range.
The difference between tris buffer and tris HCl buffer is that the former is basic while the latter is acidic. Tris HCl is basically a tris buffer solution that has had hydrochloric acid added to it.
Custom Product Development
Go from batch trials to industrial manufacturing with our product development and formulation service, led by our expert technical team.
- Free pre- and post-sales support
- Testing for consistent high quality
- Full regulatory compliance
Or call our team at 321-REAGENT
Why Choose ReAgent as Your Biological Buffers Supplier?
With almost 50 years of chemical manufacturing experience, ReAgent is a multinational biological buffers supplier that can support you with flexibility and technical expertise.
Whether you need bioprocessing reagents or custom buffer solutions, we take a collaborative approach to ensure your exact needs are met.
This might mean developing new manufacturing techniques, investing in new equipment, or even adopting industry-specific systems to meet required standards. Whatever it is, we’re happy to go that extra mile.
Face-to-face contact and support
If you have a technical question, want to talk through ideas in person, or even perform an audit, our door is always open.
Fully traceability on all batches
Our custom in-house system means we have full traceability on all stock and each batch in our facility.
Place your order, sit back, and we take care of the rest
Our service doesn’t stop when we’ve finished manufacturing your product. We can also label your products with your own labels and company-branded documents and deliver directly to you or your customers.
